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KMID : 0379120040320020095
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Korean Journal of Mycology
2004 Volume.32 No. 2 p.95 ~ p.100
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Favorable Conditions for Mycelial Growth of Phellinus linteus
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Lee Won-Ho
Park Young-Jin
Kim Tae-Woong
Sung Jae -Mo
Kim Ho-Kyoung
Kim Soo-Young
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Abstract
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The main objectives of the study were to investigate cultural characteristics of Phellinus linteus. The optimum culture media for mycelial growth of P. linteus were MYA (malt yeast agar) and SMS (soybean powder malt Sucrose). Similarly, optimum temperature and pH were $30^{\circ}C$ and 6.0, respectively. Malt extract (2%, v/v) and yeast extract (0.2%, v/v) were optimum carbon and nitrogen sources. Similarly, 0.1% $KH_2PO_4$ was optimum mineral salt. Highest mycelial growth was observed when C/N ratio was 10 : 1. Optimum inoculum amount for flask culture was $5{\sim}6$ mycelial discs (6 mm diameter) per 100 ml of liquid medium, Highest mycelial dry weight was obtained when cultured in 100 ml liquid medium in 300 ml shaking flask after 20 days of shaking culture, For mass liquid culture (8 l), flask culture was homogenized and used as an inoculum. Optimum culture period and aeration rate for 8l fermentation culture were 12 days and 2.0 vvm, respectively.
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KEYWORD
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Liquid culture, Mycelial growth, Optimum nutrients, Phellinus linteus
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